Groups of students prepare spinach “disks” for exposure to a variety of substances at different doses. The disks are placed in the exposure media and placed under UV lights which induce photosynthesis, and the disks rise to the surface as they produce oxygen. The students record how many disks have risen at 2-minute intervals and plot their results. We discuss the scientific method and hypothesis testing, the importance of replication, and the applicability of these methods for applied toxicological research in the real world. This module is best suited for students ranging from 5^th to 12^th grade with modifications to fit grade levels appropriately.

Students view zebrafish under a dissecting scope and guess what animal they are viewing. The students then guess what the developmental stage of the embryo is and verify their estimate based on photos of the fish at each possible timepoint. They learn key events in zebrafish development such as hatching, swim bladder inflation, and the development of eyes, liver, and jaw. This module is designed for students ranging from 1^st to 5^th grade.

Groups of students add varying amounts of potential “toxins” (e.g. salt or other household products) to ziplock bags containing yeast and observe the effects on yeast respiration by measuring carbon dioxide production. After, each student generates a dose-response curve by graphing the differing levels of respiration measured in each bag. Students share their dose-response curves with the class using an interactive Google sheet developed by TeamTox to display cumulative class results. The activity is followed by a discussion about dose-dependent effects of different chemicals on yeast viability, which facilitates discussion of the dose-dependent effects of chemicals on human health. This module works best for middle or high school students.

Participants extract DNA from strawberries which they can take home in a sealable vial. The experiment is accompanied by a discussion about DNA and its uses in medicine, agriculture, and toxicology. This module works best with elementary-age students.

TeamTox hosts a 3 half-day summer camp consisting of a curriculum that incorporates concepts of experimental design, environmental sampling, chemical exposure, toxicity testing, dose-response, risk assessment, and public health. Students collected their own stream samples and field observations, assessed the water quality in the lab, tested the toxicity of the water to Daphnia, and generated tables and figures (using an RShiny app developed by TeamTox) to compare results and assess risk. On the third day we use glowing zebrafish to determine molecular mechanisms of toxicity among different chemicals. We have continued to build on the TeamTox summer camp and will host a three-day camp again for the summer of 2024. Along with the experimental activities we build in time for students to ask questions about applying and attending college and what it’s like to be a scientist. This summer camp is limited to high school students.