TitleZebrafish (Danio rerio) fed vitamin E-deficient diets produce embryos with increased morphologic abnormalities and mortality.
Publication TypeJournal Article
Year of Publication2012
AuthorsMiller, GW, Labut, EM, Lebold, KM, Floeter, A, Tanguay, RL, Traber, MG
JournalJ Nutr Biochem
Volume23
Issue5
Pagination478-86
Date Published2012 May
ISSN1873-4847
Keywordsalpha-Tocopherol, Animals, Disease Models, Animal, Embryo, Nonmammalian, Female, Vitamins, Zebrafish
Abstract

Vitamin E (α-tocopherol) is required to prevent fetal resorption in rodents. To study α-tocopherol's role in fetal development, a nonplacental model is required. Therefore, the zebrafish, an established developmental model organism, was studied by feeding the fish a defined diet with or without added α-tocopherol. Zebrafish (age, 4-6 weeks) were fed the deficient (E-), sufficient (E+) or lab diet up to 1 years. All groups showed similar growth rates. The exponential rate of α-tocopherol depletion up to ~80 day in E- zebrafish was 0.029±0.006 nmol/g, equivalent to a depletion half-life of 25±5 days. From age ~80 days, the E- fish (5±3 nmol/g) contained ~50 times less α-tocopherol than the E+ or lab diet fish (369±131 or 362±107, respectively; P<.05). E-depleted adults demonstrated decreased startle response suggesting neurologic deficits. Expression of selected oxidative stress and apoptosis genes from livers isolated from the zebrafish fed the three diets were evaluated by quantitative polymerase chain reaction and were not found to vary with vitamin E status. When E-depleted adults were spawned, they produced viable embryos with depleted α-tocopherol concentrations. The E- embryos exhibited a higher mortality (P<.05) at 24 h post-fertillization and a higher combination of malformations and mortality (P<.05) at 120 h post-fertillization than embryos from parents fed E+ or lab diets. This study documents for the first time that vitamin E is essential for normal zebrafish embryonic development.

DOI10.1016/j.jnutbio.2011.02.002
Alternate JournalJ. Nutr. Biochem.
PubMed ID21684137
PubMed Central IDPMC3179832
Grant ListP30 ES000210-35 / ES / NIEHS NIH HHS / United States
HD062109 / HD / NICHD NIH HHS / United States
R01 HD062109 / HD / NICHD NIH HHS / United States
R01 HD062109-01 / HD / NICHD NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States