TitleSimultaneous detection and identification of Bacillus cereus group bacteria using multiplex PCR.
Publication TypeJournal Article
Year of Publication2007
AuthorsPark, S-H, Kim, H-J, Kim, J-H, Kim, T-W, Kim, H-Y
JournalJ Microbiol Biotechnol
Volume17
Issue7
Pagination1177-82
Date Published2007 Jul
ISSN1017-7825
KeywordsBacillus anthracis, Bacillus cereus, Bacillus thuringiensis, Base Sequence, Cloning, Molecular, DNA Primers, DNA, Bacterial, Food Microbiology, Genes, Bacterial, Molecular Sequence Data, Nucleic Acid Amplification Techniques, Oryza, Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, DNA, Time Factors, Vegetables
Abstract

Bacillus cereus group bacteria share a significant degree of genetic similarity. Thus, to differentiate and identify the Bacillus cereus group efficiently, a multiplex PCR method using the gyrB and groEL genes as diagnostic markers is suggested for simultaneous detection. The assay yielded a 400 bp amplicon for the groEL gene from all the B. cereus group bacteria, and a 253 bp amplicon from B. anthracis, 475 bp amplicon from B. cereus, 299 bp amplicon from B. thuringiensis, and 604 bp amplicon from B. mycoides for the gyrB gene. No nonspecific amplicons were observed with the DNA from 29 other pathogenic bacteria. The specificity and sensitivity of the B. cereus group identification using this multiplex PCR assay were evaluated with different kinds of food samples. In conclusion, the proposed multiplex PCR is a reliable, simple, rapid, and efficient method for the simultaneous identification of B. cereus group bacteria from food samples in a single tube.

Alternate JournalJ. Microbiol. Biotechnol.
PubMed ID18051330