TitleProteome-driven elucidation of adaptive responses to combined vitamin E and C deficiency in zebrafish.
Publication TypeJournal Article
Year of Publication2014
AuthorsMotorykin, I, Traber, MG, Tanguay, RL, Maier, CS
JournalJ Proteome Res
Volume13
Issue3
Pagination1647-56
Date Published2014 Mar 07
ISSN1535-3907
KeywordsAdaptation, Physiological, Animals, Ascorbic Acid, Ascorbic Acid Deficiency, Chromatography, Liquid, Humans, Mass Spectrometry, Metabolic Networks and Pathways, Protein Interaction Mapping, Proteome, Tissue Extracts, Vitamin E, Vitamin E Deficiency, Zebrafish
Abstract

The purpose of this study was to determine the system-wide consequences of deficiencies in two essential micronutrients, vitamins E and C, on the proteome using zebrafish (Danio rerio) as one of the few vertebrate models that similar to humans cannot synthesize vitamin C. We describe a label-free proteomics workflow to detect changes in protein abundance estimates dependent on vitamin regimes. We used ion-mobility-enhanced data-independent tandem mass spectrometry to determine differential regulation of proteins in response to low dietary levels of vitamin C with or without vitamin E. The detection limit of the method was as low as 20 amol, and the dynamic range was five orders of magnitude for the protein-level estimates. On the basis of the quantitative changes obtained, we built a network of protein interactions that reflect the whole organism's response to vitamin C deficiency. The proteomics-driven study revealed that in vitamin-E-deficient fish, vitamin C deficiency is associated with induction of stress response, astrogliosis, and a shift from glycolysis to glutaminolysis as an alternative mechanism to satisfy cellular energy requirements.

DOI10.1021/pr401108d
Alternate JournalJ. Proteome Res.
PubMed ID24476500
PubMed Central IDPMC3993953
Grant ListS10 RR025628 / RR / NCRR NIH HHS / United States
P30ES000210 / ES / NIEHS NIH HHS / United States
R01 HD062109 / HD / NICHD NIH HHS / United States
R01HD062109 / HD / NICHD NIH HHS / United States
S10RR025628 / RR / NCRR NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States