TitleNovel function of vitamin E in regulation of zebrafish (Danio rerio) brain lysophospholipids discovered using lipidomics.
Publication TypeJournal Article
Year of Publication2015
AuthorsChoi, J, Leonard, SW, Kasper, K, McDougall, M, Stevens, JF, Tanguay, RL, Traber, MG
JournalJ Lipid Res
Volume56
Issue6
Pagination1182-90
Date Published2015 Jun
ISSN1539-7262
KeywordsAnimals, Brain, Fatty Acids, Lipid Peroxidation, Lipids, Lysophospholipids, Vitamin E, Vitamin E Deficiency, Zebrafish
Abstract

We hypothesized that brains from vitamin E-deficient (E-) zebrafish (Danio rerio) would undergo increased lipid peroxidation because they contain highly polyunsaturated fatty acids, thus susceptible lipids could be identified. Brains from zebrafish fed for 9 months defined diets without (E-) or with (E+) added vitamin E (500 mg RRR-α-tocopheryl acetate per kilogram diet) were studied. Using an untargeted approach, 1-hexadecanoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine [DHA-PC 38:6, PC 16:0/22:6]was the lipid that showed the most significant and greatest fold-differences between groups. DHA-PC concentrations were approximately 1/3 lower in E- (4.3 ± 0.6 mg/g) compared with E+ brains (6.5 ± 0.9 mg/g, mean ± SEM, n = 10 per group, P = 0.04). Using lipidomics, 155 lipids in brain extracts were identified. Only four phospholipids (PLs) were different (P < 0.05) between groups; they were lower in E- brains and contained DHA with DHA-PC 38:6 at the highest abundances. Moreover, hydroxy-DHA-PC 38:6 was increased in E- brains (P = 0.0341) supporting the hypothesis of DHA peroxidation. More striking was the depletion in E- brains of nearly 60% of 19 different lysophospholipids (lysoPLs) (combined P = 0.0003), which are critical for membrane PL remodeling. Thus, E- brains contained fewer DHA-PLs, more hydroxy-DHA-PCs, and fewer lysoPLs, suggesting that lipid peroxidation depletes membrane DHA-PC and homeostatic mechanisms to repair the damage resulting in lysoPL depletion.

DOI10.1194/jlr.M058941
Alternate JournalJ. Lipid Res.
PubMed ID25855633
PubMed Central IDPMC4442875
Grant ListS10RR027878 / RR / NCRR NIH HHS / United States
HD062109 / HD / NICHD NIH HHS / United States
R01 HD062109 / HD / NICHD NIH HHS / United States
ES000210 / ES / NIEHS NIH HHS / United States
S10 RR027878 / RR / NCRR NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States