Title | Neurodevelopmental low-dose bisphenol A exposure leads to early life-stage hyperactivity and learning deficits in adult zebrafish. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Saili, KS, Corvi, MM, Weber, DN, Patel, AU, Das, SR, Przybyla, J, Anderson, KA, Tanguay, RL |
Journal | Toxicology |
Volume | 291 |
Issue | 1-3 |
Pagination | 83-92 |
Date Published | 2012 Jan 27 |
ISSN | 1879-3185 |
Keywords | Animals, Behavior, Animal, Benzhydryl Compounds, Chromatography, High Pressure Liquid, Dose-Response Relationship, Drug, Embryo, Nonmammalian, Endocrine Disruptors, Environmental Pollutants, Estradiol, Hydrazines, Hyperkinesis, Larva, Learning Disorders, Maze Learning, Phenols, Receptors, Estrogen, Receptors, G-Protein-Coupled, Reversal Learning, Teratogens, Zebrafish |
Abstract | Developmental bisphenol A (BPA) exposure has been implicated in adverse behavior and learning deficits. The mode of action underlying these effects is unclear. The objectives of this study were to identify whether low-dose, developmental BPA exposure affects larval zebrafish locomotor behavior and whether learning deficits occur in adults exposed during development. Two control compounds, 17β-estradiol (an estrogen receptor ligand) and GSK4716 (a synthetic estrogen-related receptor gamma ligand), were included. Larval toxicity assays were used to determine appropriate BPA, 17β-estradiol, and GSK4716 concentrations for behavior testing. BPA tissue uptake was analyzed using HPLC and lower doses were extrapolated using a linear regression analysis. Larval behavior tests were conducted using a ViewPoint Zebrabox. Adult learning tests were conducted using a custom-built T-maze. BPA exposure to <30μM was non-teratogenic. Neurodevelopmental BPA exposure to 0.01, 0.1, or 1μM led to larval hyperactivity or learning deficits in adult zebrafish. Exposure to 0.1μM 17β-estradiol or GSK4716 also led to larval hyperactivity. This study demonstrates the efficacy of using the zebrafish model for studying the neurobehavioral effects of low-dose developmental BPA exposure. |
DOI | 10.1016/j.tox.2011.11.001 |
Alternate Journal | Toxicology |
PubMed ID | 22108044 |
PubMed Central ID | PMC3245816 |
Grant List | P42 ES016465-04 / ES / NIEHS NIH HHS / United States R21 ES018970-02 / ES / NIEHS NIH HHS / United States T32ES7060 / ES / NIEHS NIH HHS / United States R21ES018970 / ES / NIEHS NIH HHS / United States P42 ES016465 / ES / NIEHS NIH HHS / United States P30 ES000210-44 / ES / NIEHS NIH HHS / United States R21 ES018970 / ES / NIEHS NIH HHS / United States T32 ES007060 / ES / NIEHS NIH HHS / United States P30 ES000210 / ES / NIEHS NIH HHS / United States |