TitleMicroarray detection of food-borne pathogens using specific probes prepared by comparative genomics.
Publication TypeJournal Article
Year of Publication2008
AuthorsKim, H-J, Park, S-H, Lee, T-H, Nahm, B-H, Kim, Y-R, Kim, H-Y
JournalBiosens Bioelectron
Volume24
Issue2
Pagination238-46
Date Published2008 Oct 15
ISSN0956-5663
KeywordsChromosome Mapping, DNA Probes, DNA, Bacterial, Food Analysis, Food Contamination, Food Microbiology, Oligonucleotide Array Sequence Analysis
Abstract

In order to design a method for the accurate detection and identification of food-borne pathogens, we used comparative genomics to select 70-mer oligonucleotide probes specific for 11 major food-borne pathogens (10 overlapping probes per pathogen) for use in microarray analysis. We analyzed the hybridization pattern of this constructed microarray with the Cy3-labeled genomic DNA of various food-borne pathogens and other bacteria. Our microarray showed a highly specific hybridization pattern with the genomic DNA of each food-borne pathogen; little unexpected cross-hybridization was observed. Microarray data were analyzed and clustered using the GenePix Pro 6.0 and GeneSpring GX 7.3.1 programs. The analyzed dendrogram revealed the discriminating power of constructed microarray. Each food-borne pathogen clustered according to its hybridization specificity and non-pathogenic species were discriminated from pathogenic species. Our method can be applied to the rapid and accurate detection and identification of food-borne pathogens in the food industry. In addition, this study demonstrates that genome sequence comparison and DNA microarray analysis have a powerful application in epidemiologic and taxonomic studies, as well as in the food safety and biodefense fields.

DOI10.1016/j.bios.2008.03.019
Alternate JournalBiosens Bioelectron
PubMed ID18448324