TitleIntracellular free iron and its potential role in ultrahigh-pressure-induced inactivation of Escherichia coli.
Publication TypeJournal Article
Year of Publication2013
AuthorsYan, Y, Waite-Cusic, JG, Kuppusamy, P, Yousef, AE
JournalAppl Environ Microbiol
Volume79
Issue2
Pagination722-4
Date Published2013 Jan
ISSN1098-5336
KeywordsElectron Spin Resonance Spectroscopy, Escherichia coli, Hydrostatic Pressure, Iron, Microbial Viability
Abstract

Intracellular free iron of Escherichia coli was determined by whole-cell electron paramagnetic resonance spectrometry. Ultrahigh pressure (UHP) increased both intracellular free iron and cell lethality in a pressure-dose-dependent manner. The iron chelator 2,2'-dipyridyl protected cells against UHP treatments. A mutation that produced iron overload conditions sensitized E. coli to UHP treatment.

DOI10.1128/AEM.02202-12
Alternate JournalAppl. Environ. Microbiol.
PubMed ID23124235
PubMed Central IDPMC3553779