TitleInterlaboratory evaluation of a real-time multiplex polymerase chain reaction method for identification of salmon and trout species in commercial products.
Publication TypeJournal Article
Year of Publication2011
AuthorsHellberg, RSRasmusse, Naaum, AM, Handy, SM, Hanner, RH, Deeds, JR, Yancy, HF, Morrissey, MT
JournalJ Agric Food Chem
Volume59
Issue3
Pagination876-84
Date Published2011 Feb 09
ISSN1520-5118
KeywordsAnimals, Canada, DNA, DNA Barcoding, Taxonomic, Food Contamination, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Salmon, Seafood, Trout, UNITED States
Abstract

This interlaboratory study evaluated a real-time multiplex polymerase chain reaction (PCR) method for identification of salmon and trout species in a range of commercial products in North America. Eighty salmon and trout products were tested with this method by three independent laboratories. Samples were collected in the United States and Canada, and only the collecting institution was aware of the species declaration. Following analysis with real-time PCR, all three laboratories were able to identify species in 79 of the 80 products, with 100% agreement on species assignment. A low level of fraud was detected, with only four products (5%) found to be substituted or mixtures of two species. The results for two of the fraudulent products were confirmed with alternate methods, but the other two products were heavily processed and could not be verified with methods other than real-time PCR. Overall, the results of this study show the usefulness and versatility of this real-time PCR method for the identification of commercial salmon and trout species.

DOI10.1021/jf103241y
Alternate JournalJ. Agric. Food Chem.
PubMed ID21214178