TitleGlobal gene expression analysis reveals pathway differences between teratogenic and non-teratogenic exposure concentrations of bisphenol A and 17β-estradiol in embryonic zebrafish.
Publication TypeJournal Article
Year of Publication2013
AuthorsSaili, KS, Tilton, SC, Waters, KM, Tanguay, RL
JournalReprod Toxicol
Volume38
Pagination89-101
Date Published2013 Jul
ISSN1873-1708
KeywordsAnimals, Benzhydryl Compounds, Embryo, Nonmammalian, Estradiol, Gene Expression Profiling, Gene Expression Regulation, Developmental, Oligonucleotide Array Sequence Analysis, Phenols, Teratogens, Zebrafish
Abstract

Transient developmental exposure to 0.1μM bisphenol A (BPA) results in larval zebrafish hyperactivity and learning impairments in the adult, while exposure to 80μM BPA results in teratogenic responses, including craniofacial abnormalities and edema. The mode of action underlying these effects is unclear. We used global gene expression analysis to identify candidate genes and signaling pathways that mediate BPA's developmental toxicity in zebrafish. Exposure concentrations were selected and anchored to the positive control, 17β-estradiol (E2), based on previously determined behavioral or teratogenic phenotypes. Functional analysis of differentially expressed genes revealed distinct expression profiles at 24h post fertilization for 0.1μM versus 80μM BPA and 0.1μM versus 15μM E2 exposure, identification of prothrombin activation as a top canonical pathway impacted by both 0.1μM BPA and 0.1μM E2 exposure, and suppressed expression of several genes involved in nervous system development and function following 0.1μM BPA exposure.

DOI10.1016/j.reprotox.2013.03.009
Alternate JournalReprod. Toxicol.
PubMed ID23557687
PubMed Central IDPMC3690774
Grant ListT32 ES7060 / ES / NIEHS NIH HHS / United States
P42 ES016465 / ES / NIEHS NIH HHS / United States
R21 ES018970 / ES / NIEHS NIH HHS / United States
T32 ES007060 / ES / NIEHS NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States