TitleEarly life perfluorooctanesulphonic acid (PFOS) exposure impairs zebrafish organogenesis.
Publication TypeJournal Article
Year of Publication2014
AuthorsChen, J, Tanguay, RL, Tal, TL, Gai, Z, Ma, X, Bai, C, Tilton, SC, Jin, D, Yang, D, Huang, C, Dong, Q
JournalAquat Toxicol
Volume150
Pagination124-32
Date Published2014 May
ISSN1879-1514
KeywordsAir Sacs, Alkanesulfonic Acids, Animals, Embryo, Nonmammalian, Environmental Exposure, Fluorocarbons, Gene Expression Regulation, Developmental, Intestines, Organogenesis, Water Pollutants, Chemical, Zebrafish
Abstract

As a persistent organic contaminant, perfluorooctanesulphonic acid (PFOS) has been widely detected in the environment, wildlife, and humans. The present study revealed that zebrafish embryos exposed to 16 μM PFOS during a sensitive window of 48-96 hour post-fertilization (hpf) disrupted larval morphology at 120 hpf. Malformed zebrafish larvae were characterized by uninflated swim bladder, less developed gut, and curved spine. Histological and ultrastructural examination of PFOS-exposed larvae showed structural alterations in swim bladder and gut. Whole genome microarray was used to identify the early transcripts dysregulated following exposure to 16 μM PFOS at 96 hpf. In total, 1278 transcripts were significantly misexpressed (p<0.05) and 211 genes were changed at least two-fold upon PFOS exposure in comparison to the vehicle-exposed control group. A PFOS-induced network of perturbed transcripts relating to swim bladder and gut development revealed that misexpression of genes were involved in organogenesis. Taken together, early life stage exposure to PFOS perturbs various molecular pathways potentially resulting in observed defects in swim bladder and gut development.

DOI10.1016/j.aquatox.2014.03.005
Alternate JournalAquat. Toxicol.
PubMed ID24667235
PubMed Central IDPMC4159678
Grant ListP30 ES000210 / ES / NIEHS NIH HHS / United States
P42 ES016465 / ES / NIEHS NIH HHS / United States
P30ES00210 / ES / NIEHS NIH HHS / United States