TitleDe-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast Dekkera bruxellensis AWRI1499.
Publication TypeJournal Article
Year of Publication2012
AuthorsCurtin, CD, Borneman, AR, Chambers, PJ, Pretorius, IS
JournalPLoS One
Volume7
Issue3
Paginatione33840
Date Published2012
ISSN1932-6203
KeywordsDekkera, Genome, Fungal, Haplotypes, Open Reading Frames, Phylogeny, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, Wine
Abstract

Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits.

DOI10.1371/journal.pone.0033840
Alternate JournalPLoS ONE
PubMed ID22470482
PubMed Central IDPMC3314683