TitleCaffeic acid phenethyl ester, a major component of propolis, suppresses high fat diet-induced obesity through inhibiting adipogenesis at the mitotic clonal expansion stage.
Publication TypeJournal Article
Year of Publication2014
AuthorsShin, SHo, Seo, SGwon, Min, S, Yang, H, Lee, E, Son, JEun, Kwon, JYeon, Yue, S, Chung, M-Y, Kim, K-H, Cheng, J-X, Lee, HJoo, Lee, KWon
JournalJ Agric Food Chem
Volume62
Issue19
Pagination4306-12
Date Published2014 May 14
ISSN1520-5118
KeywordsAdipocytes, Adipogenesis, Animals, Anti-Obesity Agents, Caffeic Acids, Cyclin D1, Diet, High-Fat, Down-Regulation, Humans, Male, Mice, Mice, Inbred C57BL, Mitosis, Obesity, Phenylethyl Alcohol, Propolis
Abstract

In the present study, we aimed to investigate the antiobesity effect of CAPE in vivo, and the mechanism by which CAPE regulates body weight in vitro. To confirm the antiobesity effect of CAPE in vivo, mice were fed with a high fat diet (HFD) with different concentrations of CAPE for 5 weeks. CAPE significantly reduced body weight gain and epididymal fat mass in obese mice fed a HFD. In accordance with in vivo results, Oil red O staining results showed that CAPE significantly suppressed MDI-induced adipogenesis of 3T3-L1 preadipocytes. FACS analysis results showed that CAPE delayed MDI-stimulated cell cycle progression, thereby contributing to inhibit mitotic clonal expansion (MCE), which is a prerequisite step for adipogenesis. Also, CAPE regulated the expression of cyclin D1 and the phosphorylation of ERK and Akt, which are upstream of cyclin D1. These results suggest that CAPE exerts an antiobesity effect in vivo, presumably through inhibiting adipogenesis at an early stage of adipogenesis.

DOI10.1021/jf405088f
Alternate JournalJ. Agric. Food Chem.
PubMed ID24611533