Title | Atrazine degradation by bioaugmented sediment from constructed wetlands. |
Publication Type | Journal Article |
Year of Publication | 2001 |
Authors | Runes, HB, Jenkins, JJ, Bottomley, PJ |
Journal | Appl Microbiol Biotechnol |
Volume | 57 |
Issue | 3 |
Pagination | 427-32 |
Date Published | 2001 Oct |
ISSN | 0175-7598 |
Keywords | Atrazine, Geologic Sediments, Herbicides, Oregon, Plants, Soil Microbiology, Water Pollutants, Chemical |
Abstract | The potential to establish pesticide biodegradation in constructed wetland sediment was investigated. Under microcosm conditions, bioaugmentation of sediment with small quantities of an atrazine spill-site soil (1:100 w/w) resulted in the mineralization of 25-30% of 14C ethyl atrazine (1-10 microg g(-1) sediment) as 14CO2 under both unsaturated and water-saturated conditions; atrazine and its common metabolites were almost undetectable after 30 days incubation. By comparison, unbioaugmented sediment supplemented with organic amendments (cellulose or cattail leaves) mineralized only 2-3% of 14C ethyl atrazine, and extractable atrazine and its common metabolites comprised approximately 70% of the original application. The population density of atrazine-degrading microorganisms in unbioaugmented sediment was increased from approximately 10(2)/g to 10(4)/g by bioaugmentation (1:100 w/w), and increased by another 60-fold (6.0x10(5) g(-1)) after incubation with 10 microg g(-1) of atrazine. A high population of atrazine degraders (approximately 10(6) g(-1)) and enhanced rates of atrazine mineralization also developed in bioaugmented sediment after incubation in flooded mesocosms planted with cattails (Typha latifolia) and supplemented with atrazine (3.2 mg l(-1), 1 microg g(-1) sediment). In the absence of atrazine, neither the population of atrazine degraders, nor the atrazine mineralizing potential of bioaugmented sediment increased, regardless of the presence or absence of cattails. Bioaugmentation might be a simple method to promote pesticide degradation in nursery run-off channeled through constructed wetlands, if persistence of degraders in the absence of pesticide is not a serious constraint. |
Alternate Journal | Appl. Microbiol. Biotechnol. |
PubMed ID | 11759697 |
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